Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro

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Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro

In the present studies, an attempt has been made to develop a method of liquid nitrogen preservation of plant explants propagated in vitro in the laboratory of the Department of Ornamental Plants, of Agricultural University in Kraków: shoot apical and axillary meristems of Rosa ‘New Dawn’, somatic embryos of snowdrops Galanthus nivalis L. and G. elwesii Hook, and gametophyte of Phlebodium aureu...

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Optimization of Water Content for the Cryopreservation Of Allium sativum In Vitro Cultures by Encapsulation-Dehydration.

BACKGROUND There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS Stem discs were excised from post-harvest, stored bulbs p...

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Cryopreservation of human granulocytes in liquid nitrogen.

Human granulocytes (PMNL) were successfully cryopreserved for up to 14 months. The PMNL (1-2 X 10(7)/ml) were stored in 2-ml ampoules in the gas phase of liquid nitrogen at a temperature between -160 degrees C and -196 degrees C using dimethylsulphoxide (DMSO 10%) as cryoprotectant. Morphology and phagocytic and bactericidal capacity were best preserved by adding fetal calf serum to the freezin...

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Cryopreservation of Capparis spinosa Shoot Tips via Vitrification, Encapsulation Dehydration and Encapsulation Vitrification

In vitro shoot tips of Capparis spiunosa were cryopreserved using the vitrification, encapsulation/ dehydration and encapsulation vitrification techniques. In the vitrification procedure, a maximum of 70% regrwoth was obtained when shoot tips were treated with the Plant vitrification solution (PVS2) for 60 min at 0°C and plunged directly into liquid nitrogen. In encapsulation dehydration, shoot...

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A new cryopreservation method for vegetatively propagated plant genetic resources using aluminum cryo-plates

A new cryopreservation method using aluminum cryo-plates was developed for clonally propagated crops. In this method, shoot tips are attached to the cryo-plates and can easily be manipulated for preservation treatments, such as dehydration. The ease of handling, minimal injury to shoot tips, and rapid rates of cooling and warming resulted in high regrowth rates. More than 80% regrowth was obtai...

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ژورنال

عنوان ژورنال: Folia Horticulturae

سال: 2008

ISSN: 2083-5965

DOI: 10.2478/fhort-2013-0106